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cy3 fluorescent dye  (Danaher Inc)


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    Structured Review

    Danaher Inc cy3 fluorescent dye
    Cy3 Fluorescent Dye, supplied by Danaher Inc, used in various techniques. Bioz Stars score: 93/100, based on 145 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cy3+ab+labeling+kit/Cy3+Ab+Labeling+Kit/pm37029122-248-16-19
    Average 93 stars, based on 145 article reviews
    cy3 fluorescent dye - by Bioz Stars, 2026-11
    93/100 stars

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    Related Articles

    Labeling:

    Article Title: Autonomous translocation and intracellular trafficking of the cell-penetrating and immune-suppressive effector protein YopM.
    Article Snippet: free in the cytosol and inside the nucleus.. We could not observe any colocalization of YopM with secretory membranes, which excludes retrograde transport as the mechanism for cytosolic release. however, our findings indicate that direct membrane penetration and/or an endosomal escape of YopM contribute to the cytosolic and nuclear localization of the protein.. Surprisingly, even when endocytosis is blocked, YopM was found to be associated with endosomes. this suggests an intracellular endosome-associated transport of YopM.

    Article Title: Sponge cytogenetics - mitotic chromosomes of ten species of freshwater sponge.
    Article Snippet: FISH analysis was performed as described previously (Matsuda and Chapman, 1995) using a biotin-labeled human 18S28S rDNA probe (provided by the Japanese Cancer Research Resource Bank) and digoxigenin-labeled telomere (TTAGGG)7 and (TAACCC)7 probes. .. After hybridization, the slides were incubated with FITC-avidin (Roche Diagnostics) and mouse monoclonal antidigoxigenin clone DI-22 ascites fluid (Sigma-Aldrich) labeled with Cy3 by using a Cy3 Ab Labeling Kit (Amersham Biosciences) to stain the rDNA probe and the telomere repeat probe, respectively. ..

    Article Title: Two variable lymphocyte receptor genes of the inshore hagfish are located far apart on the same chromosome.
    Article Snippet: Variable lymphocyte receptors (VLR) generate enormous diversity through assembling highly diverse leucine-rich repeat (LRR) modules and presumably function as antigen receptors in jawless vertebrates.. The hagfish, which constitute major extant members of jawless vertebrates along with lampreys, have two VLR genes designated VLRA and VLRB, whereas only a single VLR gene has been identified in the lamprey.. In the present study, we show by fluorescence in situ hybridization (FISH) that hagfish VLRA and VLRB are located on the same chromosome, but are far apart from each other.

    Article Title: Development of a novel semi-quantitative analysis system for ultramicroscale samples by fluorescent capillary isoelectric focusing.
    Article Snippet: Microchemistry provides methods to analyze small quantities of chemical substances, including proteins, nucleic acids, and carbohydrates in various fields such as biomedical research, tissue engineering, molecular biology, and regeneration medicine.. We therefore developed a fluorescent capillary isoelectric focusing (fluorescent cIEF) system for protein detection at an ultramicroscale volume, which aimed to isolate and identify, from a heterogeneous mixture of transduced cells, induced pluripotent stem cells (iPSCs) that could be fully reprogrammed.. In addition, we demonstrated that the SOX2 protein, which is indispensable for the acquisition of pluripotency, could be detected by this new fluorescent cIEF system to identify iPSCs in the early phase of complete reprogramming.

    Hybridization:

    Article Title: Sponge cytogenetics - mitotic chromosomes of ten species of freshwater sponge.
    Article Snippet: FISH analysis was performed as described previously (Matsuda and Chapman, 1995) using a biotin-labeled human 18S28S rDNA probe (provided by the Japanese Cancer Research Resource Bank) and digoxigenin-labeled telomere (TTAGGG)7 and (TAACCC)7 probes. .. After hybridization, the slides were incubated with FITC-avidin (Roche Diagnostics) and mouse monoclonal antidigoxigenin clone DI-22 ascites fluid (Sigma-Aldrich) labeled with Cy3 by using a Cy3 Ab Labeling Kit (Amersham Biosciences) to stain the rDNA probe and the telomere repeat probe, respectively. ..

    Article Title: Two variable lymphocyte receptor genes of the inshore hagfish are located far apart on the same chromosome.
    Article Snippet: Variable lymphocyte receptors (VLR) generate enormous diversity through assembling highly diverse leucine-rich repeat (LRR) modules and presumably function as antigen receptors in jawless vertebrates.. The hagfish, which constitute major extant members of jawless vertebrates along with lampreys, have two VLR genes designated VLRA and VLRB, whereas only a single VLR gene has been identified in the lamprey.. In the present study, we show by fluorescence in situ hybridization (FISH) that hagfish VLRA and VLRB are located on the same chromosome, but are far apart from each other.

    Incubation:

    Article Title: Sponge cytogenetics - mitotic chromosomes of ten species of freshwater sponge.
    Article Snippet: FISH analysis was performed as described previously (Matsuda and Chapman, 1995) using a biotin-labeled human 18S28S rDNA probe (provided by the Japanese Cancer Research Resource Bank) and digoxigenin-labeled telomere (TTAGGG)7 and (TAACCC)7 probes. .. After hybridization, the slides were incubated with FITC-avidin (Roche Diagnostics) and mouse monoclonal antidigoxigenin clone DI-22 ascites fluid (Sigma-Aldrich) labeled with Cy3 by using a Cy3 Ab Labeling Kit (Amersham Biosciences) to stain the rDNA probe and the telomere repeat probe, respectively. ..

    Article Title: Two variable lymphocyte receptor genes of the inshore hagfish are located far apart on the same chromosome.
    Article Snippet: Variable lymphocyte receptors (VLR) generate enormous diversity through assembling highly diverse leucine-rich repeat (LRR) modules and presumably function as antigen receptors in jawless vertebrates.. The hagfish, which constitute major extant members of jawless vertebrates along with lampreys, have two VLR genes designated VLRA and VLRB, whereas only a single VLR gene has been identified in the lamprey.. In the present study, we show by fluorescence in situ hybridization (FISH) that hagfish VLRA and VLRB are located on the same chromosome, but are far apart from each other.

    Staining:

    Article Title: Sponge cytogenetics - mitotic chromosomes of ten species of freshwater sponge.
    Article Snippet: FISH analysis was performed as described previously (Matsuda and Chapman, 1995) using a biotin-labeled human 18S28S rDNA probe (provided by the Japanese Cancer Research Resource Bank) and digoxigenin-labeled telomere (TTAGGG)7 and (TAACCC)7 probes. .. After hybridization, the slides were incubated with FITC-avidin (Roche Diagnostics) and mouse monoclonal antidigoxigenin clone DI-22 ascites fluid (Sigma-Aldrich) labeled with Cy3 by using a Cy3 Ab Labeling Kit (Amersham Biosciences) to stain the rDNA probe and the telomere repeat probe, respectively. ..

    Article Title: Two variable lymphocyte receptor genes of the inshore hagfish are located far apart on the same chromosome.
    Article Snippet: Variable lymphocyte receptors (VLR) generate enormous diversity through assembling highly diverse leucine-rich repeat (LRR) modules and presumably function as antigen receptors in jawless vertebrates.. The hagfish, which constitute major extant members of jawless vertebrates along with lampreys, have two VLR genes designated VLRA and VLRB, whereas only a single VLR gene has been identified in the lamprey.. In the present study, we show by fluorescence in situ hybridization (FISH) that hagfish VLRA and VLRB are located on the same chromosome, but are far apart from each other.



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    Image Search Results


    Loop design of microarray experiment . V, P, K represent Vata , Pitta , Kapha and arrow head and tail indicate Cy3 and Cy5 respectively. In one set of experiment comprising of three slides each Prakriti has been labelled with Cy3 or Cy5 and hybridised to the other two Prakriti samples labelled with the opposite dye.

    Journal: Journal of Translational Medicine

    Article Title: Whole genome expression and biochemical correlates of extreme constitutional types defined in Ayurveda

    doi: 10.1186/1479-5876-6-48

    Figure Lengend Snippet: Loop design of microarray experiment . V, P, K represent Vata , Pitta , Kapha and arrow head and tail indicate Cy3 and Cy5 respectively. In one set of experiment comprising of three slides each Prakriti has been labelled with Cy3 or Cy5 and hybridised to the other two Prakriti samples labelled with the opposite dye.

    Article Snippet: Each pool of Vata , Pitta and Kapha purified cDNA was divided into two halves, and labeled with Cy5 or Cy3 (Amersham Biosciences) using Microarray RNA Target Synthesis Kit T7 (Roche, GmbH) and the labeled products were purified using Microarray Target Purification Kit (Roche, GmbH).

    Techniques: Microarray